Flavonoid Component Determination and Apoptotic Induction Evaluation of Houttuynia cordata Thunb Extract on Human Acute Leukemic Cells
H.cordata on Human Acute Leukemic Cells
Abstract
Development of resistance to currently used drugs and side effects of several allopathic drugs have led to increased emphasis on plant materials uses as a source of medicines for a wide variety of human illnesses including leukemia. Houttuynia cordata Thunb (H.cordata), a Northern Thailand local plant and commonly known as Plucao, has various biological activities such as anti-inflammatory, anti-cancer and anti-leukemic activities. We aimed to determine active flavonoid components of H.cordata and to investigate the effect of H.cordata ethanolic extract on apoptotic induction on human acute lymphoblastic leukemia cells. In this study, we found that H.cordata ethanolic extract had total flavonoid of 231.21 ± 4.19 mg QE/g dried H.cordata. The extract analyzed by quantitative LC-MS consists of several flavonoid components including hyperin 6.35 ± 0.41, quercetin 0.34 ± 0.02, isoquercetin 1.10 ± 0.03, and rutin 0.88 ± 0.04 (%w/w). The cytotoxicity results showed dose dependent decrease in growth of Jurkat leukemic cells. Blebbing pattern of cell apoptosis was found in cells treated with H.cordata ethanolic extract for 24 and 48 hrs. Moreover, we found that the extract could substantially induce Jurkat cell death through apoptosis at both 12 and 24 hrs. In conclusion, these results indicated that H.cordata ethanolic extract which is composed of several flavonoids, possesses anti-leukemic activity through apoptotic induction in Jurkat leukemia cells.
Keywords :
acute lymphoblastic leukemia,
apoptosis,
flavonoid,
Houttuynia cordata thunb,
jurkat,
Abstrak
Kejadian ketahanan dadah masa kini dan kesan samping beberapa dadah alopati telah meningkatkan penekanan ke atas penggunaan bahan tumbuhan sebagai sumber perubatan ke atas pelbagai penyakit termasuk leukemia. Houttuynia cordata Thunb (H.cordata) ialah tumbuhan setempat di utara Thailand yang dikenali sebagai Plucao, mempunyai pelbagai aktiviti biologi seperti anti-inflamasi, anti-kanser dan anti-leukemia. Tujuan kami ialah untuk menentukan komponen aktif flavonoid H. cordata dan menyiasat kesan ekstrak etanol H.cordata ke atas induksi apoptosis pada sel leukemia limfoblastik akut manusia. Dalam kajian ini, kami mendapati ekstrak etanol H. cordata mempunyai jumlah flavonoid sebanyak 231.21 ± 4.19 mg QE/g H.cordata kering. Analisa LC-MS kuantitatif ke atas ekstrak menunjukkan ia mempunyai beberapa komponen flavonoid termasuk hiperin 6.35 ± 0.41, kuarsetin 0.34 ± 0.02, isokuarsetin 1.10 ± 0.03, and rutin 0.88 ± 0.04 (%w/w). Hasil toksisiti menunjukkan penurunan bergantung dos pada tumbesaran sel leukemia Jurkat. Corak ‘blebbing’ pada sel apotosis ditemui pada sel yang dirawat ekstrak etanol H.cordata selama 24 dan 48 jam. Tambahan lagi, kami mendapati ekstrak tersebut boleh menginduksi kematian sel Jurkat melalui apoptosis pada 12 dan 24 jam. Kesimpulannya, hasil kajian menunjukkan bahawa ekstrak etanol H.cordata yang terdiri dari beberapa flavonoid mempunyai aktiviti anti-leukemik melalui induksi apoptosis pada sel leukemia Jurkat.
Kata Kunci :
apoptosis,
flavonoid,
Houttuynia cordata Thunb,
jurkat,
leukemia limfoblastik akut,
Correspondance Address
Supantitra Chanprasert. Molecular Haematology Research Unit (MHRU), Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand 10330. E-mail: supantitra.C@chula.ac.th
