Effect of Dietary Restriction on the Healthspan of Caenorhabditis elegans
755 Effect of Dietary Restriction on Healthspan
Abstract
Dietary restriction (DR) is known to increase lifespan in Caenorhabditis elegans, but its impact on healthspan is less clear. This study investigated the effects of DR on health parameters. N2 strain nematodes (n=40) were cultured on Nematode Growth Medium (NGM) plates with E. coli, where DR was induced by diluting the bacterial concentration 100-fold. Lifespan was recorded daily, and pharyngeal pumping and locomotion were measured on days 5, 9, 13, 17 and 21. Maximum lifespan for the DR group was 28 days, compared to 25 days for the control. The decline in locomotion from day 5 to day 21 was slower in the DR group (54.7%) compared to the control (62.9%). Additionally, locomotion levels in the DR group remained consistently higher than the control throughout the study period (p<0.05). Pharyngeal pumping decreased by 62.3% in the DR group compared to 89.2% in the control group, with the DR group showing significantly higher pharyngeal pumping levels throughout the study (p<0.05). These results suggest that DR may improve functional healthspan by preserving mobility during ageing. Further studies with larger sample sizes are recommended to confirm DR’s impact on lifespan.
Keywords :
Ageing; diet; health,
Abstrak
Pembatasan diet (DR) diketahui dapat meningkatkan jangka hayat Caenorhabditis elegans, namun kesannya terhadap tempoh kesihatan kurang jelas. Kajian ini menyiasat kesan DR terhadap parameter kesihatan. Cacing N2 (n=40) dikulturkan pada plat 'Nematode Growth Medium' dengan E. coli, di mana DR diinduksi dengan mencairkan kepekatan bakteria sebanyak 100 kali. Jangka hayat direkodkan setiap hari, manakala pengepaman faring dan pergerakan diukur pada hari ke-5, 9, 13, 17 dan 21. Jangka hayat maksimum untuk kumpulan DR ialah 28 hari, berbanding 25 hari untuk kumpulan kawalan. Penurunan pergerakan dari hari ke-5 hingga hari ke-21 adalah lebih perlahan dalam kumpulan DR (54.7%) berbanding kumpulan kawalan (62.9%). Selain itu, tahap pergerakan kumpulan DR kekal lebih tinggi secara konsisten berbanding kumpulan kawalan sepanjang tempoh kajian (p<0.05). Pengepaman faring menurun sebanyak 62.3% dalam kumpulan DR berbanding 89.2% dalam kumpulan kawalan, dengan kumpulan DR menunjukkan tahap pengepaman faring yang lebih tinggi di sepanjang kajian (p<0.05). Hasil ini mencadangkan bahawa DR mungkin memperbaiki jangka kesihatan dengan memelihara mobiliti semasa penuaan. Kajian lanjut dengan saiz sampel yang lebih besar disarankan untuk mengesahkan kesan DR terhadap jangka hayat.
Kata Kunci :
Kesihatan; pemakanan; penuaan,
Correspondance Address
Jo Aan Goon. Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia. Tel: +603-91459558 Email: joaan@ukm.edu.my
