Ginger Essential Oil Inhibits Biofilm Formation of Streptococcus mutans on Stainless Steel Placeholder
Effect of Ginger Essential Oil on Biofilm
Abstract
Streptococcus mutans (S. mutans) forms biofilm on dental apparatus and materials in the mouth as far as the tooth surface is concerned. This form of biofilm around the apparatus can cause decay in the teeth. In our study, the formation of biofilm on the stainless-steel space maintainer, which is frequently used in children, and its inhibition by ginger essential oil were examined. Minimum inhibitory concentration (MIC) assays for the antibacterial activities of the ginger essential oil was performed according to the microdilution broth method. The formation of the biofilm on the stainless steel space maintainer was evaluated spectrophotometrically and the number of bacteria in the biofilm was counted. Biofilm on stainless steel space maintainer was also examined with scanning electron microscopy (SEM). On the stainless steel space maintainer without ginger essential oil, S. mutans produced significant amounts of biofilm. Bacterial counts and SEM images also supported this finding. Ginger (Zingiber officinale) essential oil significantly inhibited the formation of biofilm and adhesion of S. mutans on the stainless-steel placeholder.
Keywords :
antibiofilm activity,
caries,
ginger,
Streptococcus mutans,
Zingiber officinale (Roscoe) essential oil,
Abstrak
Streptococcus mutans (S. mutans) membentuk biofilm pada peralatan dan bahan pergigian dalam mulut iaitu permukaan gigi. Pembentukan biofilm ini boleh menyebabkan pereputan gigi. Dalam kajian ini, pembentukan biofilm pada penyelenggara ruang keluli tahan karat, yang sering digunakan oleh kanak-kanak dan perencatan oleh minyak pati halia telah dikaji. Asai kepekatan perencat minimum (MIC) dijalankan untuk mengkaji aktiviti antibakterial minyak pati halia mengikut kepada teknik larutan broth. Pembentukan biofilm pada penyelenggara ruang keluli tahan karat dinilai dengan menggunakan spektrofotometer dan bilangan bakteria dalam biofilm telah dihitung. Biofilm pada penyelenggara ruang keluli tahan karat juga dikaji dengan menggunakan mikroskop elektron pengimbas (SEM). Pada penyelenggara ruang keluli tahan karat yang tidak mempunyai minyak pati halia, S. mutans menghasilkan lebih banyak biofilm. Bilangan bakteria dan gambar SEM juga menyokong dapatan ini. Minyak pati halia (Zingiber officinale) menghalang pembentukan biofilm dan pelekatan S. mutans pada pemegang tempat keluli tahan karat.
Kata Kunci :
aktiviti antibiofilem,
halia,
karies,
minyak pati Zingiber officinale (Roscoe) Streptococcus mutans,
Correspondance Address
Prof. Dr. Merih Kıvanç. Eskişehir Technical University, Science of Faculty, Department of Biology, Eskişehir, Turkey. Tel: +90 5059235003 Email: mkivanc@eskisehir.edu.tr
