Antidiabetic Effect of Actinodaphne angustifolia and Profiling of Bioactive Metabolites using UPLC-QToF/ESI-MS Method
Antidiabetic effect of Actinodaphne angustifolia
Abstract
Diabetes mellitus is a chronic disorder that causes elevated blood glucose levels due to a lack of insulin, either completely or partially. We investigated the antidiabetic property of Actinodaphne angustifolia in a rat model and identified the bioactive phytochemicals by using the UPLC-QTOF/ESI-MS method. The rats’ pancreatic structures, lipid profile and blood glucose were assessed after a one-week intervention. UPLC-QTOF/ESI-MS analysis was conducted to identify flavonoids and terpenoids in the leaf extract. Actinodaphne angustifolia extract markedly increased the high-density lipoprotein (HDL) (p<0.05) while reducing total cholesterol (TC), low-density lipoprotein (LDL) and blood glucose. Furthermore, the tissue architecture of pancreatic islets was also well recovered as compared to the control group. A total of 45 flavonoids and 109 terpenoid compounds were identified using UPLC-QTOF/ESI-MS-based analysis and additional studies should be undertaken to identify the potential antidiabetic agents.
Keywords :
Actinodaphne angustifolia,
antidiabetic activity,
phytochemical,
UPLC-QTOF/ESI-MS,
Abstrak
Diabetes mellitus adalah gangguan kronik yang menyebabkan paras glukosa darah meningkat akibat kekurangan insulin, sama ada sepenuhnya atau sebahagian. Kami menyiasat sifat antidiabetik Actinodaphne angustifolia dalam model tikus dan mengenal pasti fitokimia bioaktif dengan menggunakan kaedah UPLC-QTOF/ESI-MS. Struktur pankreas tikus, profil lipid dan glukosa darah dinilai selepas intervensi selama satu minggu. Analisis UPLC-QTOF/ESI-MS telah dijalankan untuk mengenal pasti flavonoid dan terpenoid dalam ekstrak daun. Ekstrak Actinodaphne angustifolia dengan ketara meningkatkan lipoprotein berketumpatan tinggi (HDL) (p<0.05) sambil mengurangkan jumlah kolesterol (TC), lipoprotein berketumpatan rendah (LDL) dan glukosa darah. Tambahan pula, struktur seni tisu pulau pankreas juga pulih dengan baik berbanding kumpulan kawalan. Sebanyak 45 flavonoid dan 109 sebatian terpenoid telah dikenal pasti menggunakan analisis berasaskan UPLC-QTOF/ESI-MS dan kajian tambahan perlu dijalankan untuk mengenal pasti agen antidiabetik yang berpotensi.
Kata Kunci :
Actinodaphne angustifolia,
aktiviti antidiabetik,
fitokimia,
UPLC-QTOF/ESI-MS,
Correspondance Address
AKM Moyeenul Huq. Bio Aromatic Research Centre, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang, Malaysia. Tel: +6018-2620051 Email: moyeenul@ump.edu.my; moyeenul.rph@uap-bd.edu/ Wan Maznah Wan Ishak. Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang, Malaysia. Tel: +6017-7142780 E-mail: wanmaznah@ump.edu.my
