Gamma-tocotrienol Alters Protein Expression of HepG2 Cell Line
Gamma-tocotrienol Alters Protein Expression of HepG2 Cell Line
Abstract
Gamma-tocotrienol (GTT) has been shown to exhibit significant antitumor activity in a variety of tumor cells. Previous findings have demonstrated that GTT had antiproliferative effects on a liver cancer cell line (HepG2) with an IC50 value of 170μM. In this study, two dimensional gel electrophoresis (2DE) was used to determine changes in protein expression in HepG2 cell line following treatment with GTT. The ultimate aim is to identify the possible molecular mechanisms involved in GTT antitumor activity. This study is focused on obtaining a 2DE protein profile for HepG2 cell line with and without GTT treatment. In the preliminary analysis of the resulting 2DE profiles, 18 protein spots were found to be differentially expressed in cells treated with GTT. This observation is confirmed by extending the analysis to a larger sample size. By studying the effects of GTT treatment on differential protein expression in HepG2 cells the underlying mechanisms involved in the antitumor activity of GTT may be elucidated.
Keywords :
Gamma-tocotrie-nol (GTT),
HepG2 cells,
Proteomics,
two dimensional gel electrophoresis (2DE),
Abstrak
Gamma-tokotrienol (GTT) telah menunjukkan aktiviti antitumor yang signifikan terhadap pelbagai sel tumor. Penemuan terdahulu menunjukkan bahawa GTT mempunyai kesan antiproliferasi terhadap sel kanser hepar (HepG2) pada nilai IC50 170 μM. Di dalam kajian ini, kaedah elektroforesis gel dua dimensi (2DE) digunakan bagi mengetahui perubahan pada ekspresi protein di dalam sel HepG2 selepas rawatan GTT. Tujuan utamanya adalah untuk mengenalpasti mekanisme molekul yang mungkin terlibat dalam aktiviti antitumor GTT. Penumpuan diberikan kepada penghasilan profil protein 2DE sel HepG2 dengan dan tanpa rawatan GTT. Analisis awal terhadap profil 2DE, menunjukkan terdapat 18 titik protein diekspreskan secara berlainan di dalam sel dirawat GTT. Pemerhatian ini dipastikan dengan meluaskan lagi skop kajian kami kepada saiz sampel yang lebih besar. Dengan mengkaji kesan rawatan GTT kepada ekspresi protein di dalam sel HepG2, mekanisme asas yang terlibat pada sifat anti tumor secara diferensial GTT mungkin boleh dijelaskan.
Kata Kunci :
elektroforesis gel dua dimensi (2DE),
Gamma-tokotrienol (GTT),
Proteomik,
sel HepG2,
Correspondance Address
Dr Zakiah Jubri @ Mohd Zufri, Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia Kuala Lumpur, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Email: zakiah@medic.ukm.my
