Effects of 11β-Hydroxysteroid Dehydrogenase Type 1 Enzyme in the Liver in Fructose Induced Metabolic Syndrome Rat Model
11β-Hydroxysteroid Dehydrogenase Enzyme in Metabolic Syndrome
Abstract
Excessive expression and activity of 11β-hydroxysteroid dehydrogenase enzyme type 1 (11β-HSD1) enzyme in mature adipocyte leads to obesity and metabolic syndrome. Fructose in drinking water was proven to induce metabolic syndrome in male Wistar rat. Hence, the aim of the study was to assess the effects of expression and activity of 11β-HSD1 enzyme in the liver in an established metabolic syndrome rat model induced by fructose drinking water. Twelve male Wistar rats were randomly divided into two groups: Control group, C (n=6) and Fructose drinking water 20%, F20 (n=6). The food and fluid intake were given as ad libitum for eight weeks. At the end of the experiment, the expression of 11β-HSD1 enzyme in the liver was measured by immunohistochemical staining method. The score was given according to the intensity of the staining of the granules in the hepatocyte cytoplasm which was measured using double-blinded method. Meanwhile, the activity of 11β-HSD1 enzyme in the liver was measured using ELISA technique. Following eight weeks of consumption of fructose drinking water, the F20 group showed an increased in both expression and activity of 11β-HSD1 in the liver. The obtained data clearly suggest that 11β-HSD1 enzymes in the liver may play a role in the development of metabolic syndrome and its complications in male Wistar rat.
Keywords :
11β-hydroxysteroid dehydrogenase,
fructose,
metabolic syndrome,
Wistar rat,
Abstrak
Peningkatan ekspresi dan aktiviti enzim 11β-hidroksisteroid dehidrogenase jenis 1 (11β-HSD1) di dalam sel adiposit matang menyebabkan obesiti dan sindrom metabolik. Fruktos dalam air minuman telah terbukti boleh menyebabkan sindrom metabolik pada tikus Wistar jantan. Oleh itu, kajian ini dilakukan untuk melihat kesan ke atas ekspresi dan aktiviti enzim 11β-HSD1 di dalam hati model tikus sindrom metabolik yang dirangsang dengan air minuman fruktos. Sebanyak 12 ekor tikus Wistar jantan dibahagikan secara rawak kepada dua kumpulan: kumpulan kawalan, C (n=6) dan kumpulan yang diberi minuman fruktos 20%, F20 (n=6). Pemberian makanan dan air minuman selama lapan minggu secara ad libitum. Di akhir kajian, pengukuran ekspresi enzim 11β-HSD1 di dalam hati dilakukan dengan menggunakan teknik pewarnaan imunohistokimia. Skor diberikan berdasarkan intensiti pewarnaan granul di dalam sitoplasma hepatosit menggunakan teknik ‘double-blinded’. Manakala, aktiviti enzim 11β-HSD1 diukur menggunakan teknik ELISA. Selepas lapan minggu pengambilan air minuman fruktos, kumpulan F20 menunjukkan peningkatan dalam ekspresi dan aktiviti enzim 11β-HSD1 di dalam hati. Data yang diperolehi menunjukkan bahawa enzim 11β-HSD1 di dalam hati mungkin memainkan peranan dalam pembentukan sindrom metabolik dan komplikasinya pada tikus Wistar jantan.
Kata Kunci :
11β-hidroksisteroid dehidrogenase,
fruktos,
sindrom metabolik,
tikus Wistar,
Correspondance Address
Farihah Suhaimi, Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia. Tel: +603 91458639/8605 Fax: +603 91458607 E-mail: farihah@ppukm.ukm.edu.my/ farihah_suhaimi@yahoo.com
