The objective of this research endeavour was to ascertain whether there existed a distinction in the protein expression levels of phosphatase and tensin homolog (PTEN), phosphatidylinositol-4,5-biphosphate3-kinase (PI3K), macrophage (CD68), and interleukin-6 (IL-6) among women who had been diagnosed as having ovarian carcinoma (endometrioid and clear cell subtypes) underlying endometriosis. Tissue microarray (TMA) slides were fabricated using donor’s formalin-fixed paraffin-embedded (FFPE) blocks that comprised specimens of ovarian carcinomas tissues with the subtypes of endometrioid and clear cell that were retrieved for a duration of nine years. A total of 19 FFPE blocks represented 19 cases of carcinoma of the ovary, which were categorised into two groups: ovarian cancer with endometriosis (EAOC, n=10) and ovarian cancer without endometriosis (n=9). All of these protein expressions were analysed using immunohistochemistry (IHC) technique. Following that, comparisons were made regarding the clinical and pathological characteristics of the ovarian carcinomas, as well as any discernible variations in expression levels between the two groups. In EAOC, PTEN expression was lower (88.9% vs. 100%, P=0.47) compared to those without endometriosis. Our findings revealed an increase of PI3K and IL-6 in EAOC than those devoid of endometriosis; 80% vs. 77.8%, P=1.00 and 70% vs 11.11%, P=0.35, respectively. In contrast, CD68 was significantly lower in endometriosis-associated ovarian cancer compared to those without endometriosis (40% vs. 66.67%, P=0.16). In conclusion, there were no significant difference in the mutational and inflammatory marker modifications between clear cell carcinoma and endometrioid adenocarcinoma of ovary with endometriosis.
PTEN and PI3K Mutation Markers and Expression of CD68 and IL-6 Inflammatory Markers in Endometrioid and Clear Cell Ovarian Carcinoma with Underlying Endometriosis
Author
Abstrak
Objektif penyelidikan ini adalah untuk memastikan sama ada terdapat perbezaan dalam tahap ekspresi protein fosfatase dan tensin homolog (PTEN), fosfatidilinositol-4,5-bifosfat3-kinase (PI3K), makrofaj (CD68) dan interleukin-6 (IL-6) di kalangan wanita yang telah didiagnosis menghidap karsinoma ovari (subjenis endometrioid dan sel jernih) yang mendasari endometriosis. Slaid tisu mikrorai (TMA) telah disediakan dengan menggunakan blok parafin terfiksasi formalin (FFPE) penderma yang mengandungi spesimen tisu karsinoma ovari dengan subtaip endometrioid dan karsinoma sel jernih pada ovari yang telah diambil untuk tempoh sembilan tahun. Sebanyak 19 blok FFPE terdiri daripada 19 kes kansasinoma ovari, yang dikategorikan kepada dua kumpulan iaitu kanser ovari dengan endometriosis (EAOC, n=10) dan kanser ovari tanpa endometriosis (n=9). Kesemua ekspresi protein ini telah dianalisis menggunakan teknik imunohistokimia (IHC). Setelah itu, perbandingan berkaitan dengan ciri-ciri klinikal dan patologi karsinoma ovari, serta sebarang variasi yang boleh dilihat dalam tahap ekspresi antara kedua-dua kumpulan telah dilakukan. Dalam EAOC, ekspresi PTEN adalah lebih rendah (88.9% berbanding 100%, P=0.47) berbanding mereka yang tidak mempunyai endometriosis. Penemuan kami mendedahkan peningkatan PI3K dan IL-6 dalam EAOC daripada mereka yang tidak mempunyai endometriosis; masing-masing terdapat 80% berbanding 77.8%, P=1.00 dan 70% berbanding 11.11%, P=0.35. Sebaliknya, CD68 adalah jauh lebih rendah dalam kanser ovari yang berkaitan dengan endometriosis berbanding mereka yang tidak mempunyai endometriosis (40% berbanding 66.67%, P=0.16). Kesimpulannya, terdapat perbezaan ketara dalam pengubahsuaian penanda mutasi dan keradangan antara karsinoma sel jernih dan adenokarsinoma endometrioid ovari dengan endometriosis.
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