The tumour suppressor gene p53 and the proto-oncogene Bcl-2 encode respectively, for a nuclear phosphoprotein and for a mitochondrial membrane protein involved in multiple cellular functions. Both proteins are linked to programmed cell death pathways and provide prognostic information on breast carcinoma. Our aim is to study the expression of p53 and Bcl-2 oncoproteins in breast carcinoma and correlate with patients’ age, tumour size, disease stage and histological grade. Fifty nine cases of breast carcinomas from Universiti Kebangsaan Malaysia Medical Centre (UKMMC) were studied with the immunohistochemical method. Our results showed 45.8% (27 of 59) and 40.7% (24 of 59) of the breast carcinomas were immunopositive for p53 and Bcl-2 respectively. There was significant correlation between Bcl-2 expression with early tumour stage (p=0.01). No significant relationship was seen with other variables. Results also showed an inverse relationship between p53 and Bcl-2 expression (p=0.001). These findings indicate a down regulation of Bcl-2 by p53 in breast carcinogenesis.
Inverse Correlation between P53 and Bcl-2 Expression in Breast Carcinoma of Malaysian Patients
Author
Abstrak
Gen penindas p53 dan proto-onkogen Bcl-2 mengkodkan fosfoprotin nuklear dan protin membran mitokondria yang terlibat dalam pelbagai fungsi sel. Kedua-dua protin mempunyai hubung kait dengan tapak jalan kematian sel berprogram disamping memberi informasi prognostik karsinoma payudara. Objektif kajian ini adalah untuk menentukan kaitan onko-protin p53 dan Bcl-2 pada karsinoma payudara dan hubungannya dengan umur pesakit, saiz tumor, peringkat kanser dan gred histologi. Lima puluh sembilan kes kanser payudara dari Pusat Perubatan Universiti Kebangsaan Malaysia (PPUKM) telah dikaji menggunakan teknik imunohistokimia. Keputusan menunjukkan 45.8% (27/59) kanser payudara adalah imunopositif untuk p53 dan 40.7% (24/59) adalah imunopositif untuk Bcl-2. Terdapat korelasi signifikan di antara ekspresi Bcl-2 dengan peringkat awal kanser (p=0.01). Variabel lain tidak menunjukkan kaitan signifikan. Kaitan songsang didapati di antara p53 dan Bcl-2 (p=0.001). Hasil keputusan mencadangkan regulasi menurun Bcl-2 oleh p53 dalam karsinogenesis kanser payudara.
Kata kunci
Abstract
Keywords
|
|
|
Related Images
