The tetraspanin gene, CD151 is involved in various tumour cell progression and metastasis. Its expression is increased in high grade, estrogen receptor negative and c-erbB-2 positive breast cancer. However, the biological function and expression phenotype among different tumour status, estrogen receptor (ER) status, progesterone receptor (PR) status and c-erbB-2 expression in multi-ethnic Malaysian breast cancer patients has not been well investigated. We used quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) to measure the CD151 gene expression in 45 breast cancers. Our preliminary results revealed that CD151 expression is significantly higher in ER positive and PR positive breast cancers at 95% and 99% confidence intervals, respectively. In contrast, there is no significant correlation between CD151 expression and tumour grades or c-erbB-2 status at 95% confidence interval level. Our preliminary findings suggested that CD151 may be involved in the estrogen responsive pathways. CD151 could be a potential prognostic marker and therapeutic target in the treatment of estrogen dependent breast cancer patients.
Elevated Expression of CD151 Gene in Estrogen Receptor and Progesterone Receptor Positive Breast Carcinoma
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Abstrak
CD151 adalah gen tetraspanin yang terlibat dalam perkembangan dan perebakan sel- sel barah. Ekspresi gen ini meningkat pada kanser payudara gred tinggi, reseptor es- trogen negatif dan c-erbB-2 positif. Walaubagaimanapun, fungsi biologi dan fenotaip di kalangan pesakit yang mempunyai status barah yang berbeza, status reseptor estro- gen (ER), status reseptor progesteron (PR) dan ekspresi c-erbB-2 di Malaysia yang terdiri daripada pelbagai kaum, masih belum dikaji secara teliti. Kami menggunakan teknik real-time polymerase chain reaction (qRT-PCR) kuantitatif untuk mengukur eks- presi gen CD151 bagi 45 kanser payudara. Hasil kajian menunjukkan bahawa ekspresi CD151 adalah tinggi pada kanser payudara ER positif (95% interval konfiden) dan PR positif (99% interval konfiden). Sebaliknya, tiada korelasi didapati antara ekpresi CD151 dengan gred tumor atau status c-erbB-2 pada 95% interval konfiden. Hasil kajian setakat ini mencadangkan bahawa CD151 berpotensi sebagai penanda prognostik dan sasaran terapeutik untuk rawatan pesakit payudara estrogen dependen.
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