Three dimensional (3D) models mimic the features of native tissue environment.
Thus, morphology and signalling of cells from 3D culture are often more
physiological than routine two dimensional (2D) cell culture. It is also known
that the cell-secreted products have paracrine effect on other cells growth. In
this experimental study, we optimised the nasal fibroblast culture on a 3D cell
culture system and studied the effects of secretome from the 3D culture (3DCM)
onto fibroblast growth and cytoprotection. Nasal fibroblast was isolated from
human nasal turbinates. The suitable microcarrier was selected by culturing the
fibroblasts in passage 3 on various types PolyGEM™ polystyrene microcarriers.
Then, the cells were cultured on selected microcarrier using a 3D culture system
and the conditioned medium (CM) was collected. 3DCM were supplemented to
fibroblasts to study for attachment, proliferation, and cytoprotective effects against
cytotoxicity of Centella asiatica. Bicinchonic Acid Assay (BCA) was performed
to quantify protein amount in CMs. Sodium dodecyl sulphate-polyacrylamide
gel electrophoresis (SDS-PAGE) was performed for preliminary profiling and
comparison of 2DCM and 3DCM protein profile. Our study showed that the 3DCM
did not significantly enhance cell attachment and proliferation. The secretome
of both 2DCM and 3DCM found to have significant cytoprotective effect onto
nasal fibroblast against cytotoxicity of C.asiatica extract. 3DCM had higher protein
concentration than 2DCM. SDS-PAGE showed three exclusive proteins in 3DCM
and four exclusive proteins in 2DCM. Future study should be conducted on
utility of nasal fibroblast secretome on cytoprotection against harmful agents in
environment and cytotoxicity of natural products.