Gelam honey was reported to exhibit anti-oxidative, anti-tumour and anti-inflammatory properties against many types of cancer. The main aim of the present study was to evaluate the effect of Gelam honey on the oxidative stress level of human lung cancer cells. IC50 of Gelam honey was identified by treating A549 cells with different doses of honey (50-200 mg/ml). The cells were divided into four groups and induced with stress using hydrogen peroxide (H2O2) accordingly: control, H2O2, Gelam honey, H2O2 + Gelam honey. After 24 hrs of treatment, oxidative stress markers such as malondialdehyde (MDA) and protein carbonyl, were determined. Induction of oxidative stress significantly increased the levels of MDA (p<0.05) but had no effect on protein carbonyl levels. Treatment with Gelam honey was found to reduce MDA levels (p<0.05) in A549 cells exposed to H2O2 but had no effect on the level of protein carbonyl. Interestingly, Gelam honey treatment alone had no effects on the levels of MDA and protein carbonyl. In conclusion, Gelam honey reduces lipid peroxidation but not protein oxidation in human lung cancer cells subjected to oxidative stress.
Effects of Gelam Honey on Oxidative Stress in Lung Cancer Cells
Abstrak
Madu gelam telah didapati mempunyai kesan anti-oksidatif, anti-kanser dan anti-inflamasi terhadap banyak jenis kanser. Tujuan kajian ini adalah untuk menentukan kesan madu gelam terhadap aras tekanan oksidatif sel kanser peparu manusia. IC50 madu gelam ditentukan dengan merawat sel A549 dengan dos madu yang berbeza (50-200 mg/ml). Sel dibahagikan kepada 4 kumpulan dan diaruh tekanan oksidatif dengan menggunakan hidrogen peroksida (H2O2) mengikut kumpulan tertentu: kawalan, H2O2, madu gelam, H2O2 + madu gelam. Selepas 24 jam rawatan, biopetanda tekanan oksidatif seperti malondialdehid (MDA) dan protein karbonil ditentukan. Aruhan tekanan oksidatif meningkatkan aras MDA (p<0.05) tetapi tidak memberi kesan kepada protein karbonil. Rawatan madu gelam didapati menurunkan aras MDA (p<0.05) dalam sel A549 yang diaruh dengan H2O2 tetapi tidak memberi kesan kepada protein karbonil. Rawatan madu gelam sahaja tidak mempunyai kesan terhadap aras MDA dan protein karbonil. Kesimpulannya, madu gelam menurunkan peroksidasi lipid tetapi bukan oksidasi protein dalam sel peparu manusia teraruh tekanan oksidatif.
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